منابع مشابه
Microtubule dynamics in interphase cells
The sites of microtubule growth and the kinetics of elongation have been studied in vivo by microinjection of biotin-labeled tubulin and subsequent visualization with immunocytochemical probes. Immunofluorescence and immunoelectron microscopy demonstrate that injected biotin-labeled subunits are incorporated into new segments of growth which are contiguous with unlabeled microtubules. Rapid inc...
متن کاملInterphase microtubule dynamics are cell type-specific.
The rate and pattern of microtubule polymer loss in interphase cells have been examined using nocodazole to block microtubule assembly. Cells were incubated with high concentrations of nocodazole for various times and the pattern of microtubule disassembly was determined using tubulin immunofluorescence. Polymer loss was quantitated by measuring the decrease in percentage of cell area occupied ...
متن کاملIntrinsic microtubule stability in interphase cells
Interphase microtubule arrays are dynamic in intact cells under normal conditions and for this reason they are currently assumed to be composed of polymers that are intrinsically labile, with dynamics that correspond to the behavior of microtubules assembled in vitro from purified tubulin preparations. Here, we propose that this apparent lability is due to the activity of regulatory effectors t...
متن کاملAugmin Triggers Microtubule-Dependent Microtubule Nucleation in Interphase Plant Cells
Microtubule (MT)-dependent MT nucleation by γ-tubulin is required for interphase plant cells to establish a highly dynamic cortical MT network underneath the plasma membrane, which influences the deposition of cell wall materials and consequently governs patterns of directional cell expansion. Newly formed MTs either assume 40° angles or are parallel to the extant ones. To date, it has been eni...
متن کاملIntegrin-Linked Kinase Regulates Interphase and Mitotic Microtubule Dynamics
Integrin-linked kinase (ILK) localizes to both focal adhesions and centrosomes in distinct multiprotein complexes. Its dual function as a kinase and scaffolding protein has been well characterized at focal adhesions, where it regulates integrin-mediated cell adhesion, spreading, migration and signaling. At the centrosomes, ILK regulates mitotic spindle organization and centrosome clustering. Ou...
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ژورنال
عنوان ژورنال: The Journal of Cell Biology
سال: 1986
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.102.3.1020